Localization and Identification of Peptides from Tissue using high-speed MALDI TOF/TOF mass spectrometry

نویسندگان

  • Michael Becker
  • Anja Resemann
  • Janine Beckmann
  • Rainer Paape
  • Detlev Suckau
چکیده

MALDI Imaging is an analytical method for the detection of potential biomarkers directly from tissue sections that has gained popularity over the past decade. The development protocols for spatially resolved digests are of particular interest. Most importantly, on-tissue digestion allows the analysis of peptides from formalin-fixed paraffin-embedded (FFPE) tissue, the most common type of sample in clinical pathology. In addition, it allows detection of larger proteins using peptides as proxies and facilitates biomarker ID by means of MS/MS.

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تاریخ انتشار 2016