Localization and Identification of Peptides from Tissue using high-speed MALDI TOF/TOF mass spectrometry
نویسندگان
چکیده
MALDI Imaging is an analytical method for the detection of potential biomarkers directly from tissue sections that has gained popularity over the past decade. The development protocols for spatially resolved digests are of particular interest. Most importantly, on-tissue digestion allows the analysis of peptides from formalin-fixed paraffin-embedded (FFPE) tissue, the most common type of sample in clinical pathology. In addition, it allows detection of larger proteins using peptides as proxies and facilitates biomarker ID by means of MS/MS.
منابع مشابه
On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry.
MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for the detection and localization of drugs, proteins, and lipids on-tissue. Nevertheless, this approach can only perform identification of low mass molecules as lipids, pharmaceuticals, and peptides. In this article, a combination of approaches for the detection and imaging of proteins and their identification directly on-t...
متن کاملIdentification of Candida species isolated from vulvovaginitis in Mashhad, Iran by Use of MALDI-TOF MS
Background and Purpose Vulvovaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identify of Candida species isolated from vulvovaginitis woman suffering vulvovaginitis refered to Ghaem Hospital, Mashhad, Iran, by use of MALDI-TOF mass spectrometry. Materials and Methods The 65 clinical samples isolated from Vulvovaginitis women were collected in...
متن کاملSelective isolation of glycoproteins and glycopeptides for MALDI-TOF MS detection supported by magnetic particles.
Glycosylation is the most common form of posttranslational modification of proteins (50-80%). The isolation, discovery, and subsequent identification of glycosylated peptides and proteins is becoming more and more important in glycoproteomics and diagnosis. MALDI-TOF mass spectrometry is an ideal technique for identifying peptides and proteins and their corresponding modifications. The enrichme...
متن کاملTwo different Methods in Protein Identification by Mass Spectrometry
There are tow major methods that are widely used for protein identification by mass spectrometry: MALDI-TOF based protein fingerprinting and LC-MS/MS based peptide sequencing. In the MALDI-TOF based protein fingerprinting method, a sample is digested with certain proteolytic enzyme (usually trypsin) and one MS spectrum is acquired which generates the massed of all peptides (or MH+), and these m...
متن کاملHigh sensitivity identification of membrane proteins by MALDI TOF-MASS spectrometry using polystyrene beads.
Membrane proteins play a large variety of functions in life and represent 30% of all genomes sequenced. Due to their hydrophobic nature, they are tightly bound to their biological membrane, and detergents are always required to extract and isolate them before identification by mass spectrometry (MS). The latter, however remains difficult. Peptide mass fingerprinting methods using techniques suc...
متن کامل